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kore biotech tissue culture

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kore biotech tissue culture

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What is Tissue Culture?

The propagation of a plant by using a plant part or single cell or group cell in a test tube under very controlled and hygienic conditions is called "Tissue Culture".

What are advantages of Tissue Culture Planting Material ?

  • True to the type of mother plant under well management.
  • Pest and disease free seedlings.
  • Uniform growth, increases yield.
  • Early maturity of crop - maximum land use is possible in low land holding country like India.
  • Round the year planting possible as seedlings are made available throughout the year.
  • Two successive ratoons are possible in a short duration which minimizes cost of cultivation.
  • No staggered harvesting.
  • 95% - 98% plants bear bunches.
  • New varieties can be introduced and multiplied in a short duration.

Which is the Planting Material ?

Sword suckers weighing approximately 500-1000 gm are commonly used as propagating material. Suckers generally may be infected with some pathogens and nematodes. Similarly due to the variation in age and size of sucker the crop is not uniform, harvesting is prolonged and management becomes difficult. Therefore, in-vitro clonal propagation i.e. Tissue culture plants are recommended for planting. They are healthy, disease free, uniform and authentic. Properly hardened secondary seedlings are only recommended for planting

Which Climate is suitable for banana plantations ?

Banana is basically a tropical crop, grows well in temperature range of 13ºC – 38ºC with RH regime of 75-85%. In India this crop is being cultivated in climate ranging from humid tropical to dry mild subtropics through selection of appropriate varieties like Grandnaine. Chilling injury occurs at temperatures below 12ºC. The normal growth of the banana begins at 18ºC, reaches optimum at 27ºC, then declines and comes to a halt at 38ºC. Higher temperature causes sun scorching. High velocity wind which exceeds 80 km phrs damages the crop.

How should be the Soil?

Soil for banana should have good drainage, adequate fertility and moisture. Deep, rich loamy soil with pH between 6-7.5 is most preferred for banana cultivation. Ill drained, poorly aerated and nutritionally deficient soils are not suitable for banana. Saline solid, calcareous soil is not suitable for Banana cultivation. Avoided soil of low laying areas, very sandy & heavy black cotton with ill drainage.
A soil that is not too acidic & not too alkaline, rich in organic material with high nitrogen content, adequate phosphorus level and plenty of potash are good for banana

What is the right Planting Time?

Planting of tissue culture Banana can be done throughout the year except when the temperature is too low or too high. Facility of drip irrigation system is important. There are two important seasons in Maharashtra, India;
Mrig Baug (Kharif) Month of planting June - July.Kande Baug (Rabi) Month of planting October -November.


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